Development and Validation of New UV-Spectrophotometric Method for The Quantitation of Retigabine In Pure and Formulations

Abstract

A simple, accurate, precise, economical and reproducible UV-spectrophotometric method has been developed for the simultaneous estimation of Retigabine in bulk and in combined tablet dosage form. The stock solutions were prepared in methanol followed by the further required dilutions with distilled water. This method involves the formation and solving of simultaneous equations at 254 nm as absorbance maxima of Retigabine respectively. Linearity obeyed the concentration range of 25µg/mL to 75µg/mL for Retigabine respectively. The results of analysis were validated statistically and by recovery studies. The % RSD for the recovery study was less than 2.0. The proposed method can be effectively applied for the estimation of Retigabine in bulk and in combined tablet dosage form