A New Simple Validated RP-HPLC-PDA Analytical Method for Estimation of Aceclofenec in Active Pharmaceutical Ingredients and Bulk Drug Form Used as Anti-Anti-Inflammatory Drug

Authors

  • Sachin S. Garad
  • Dhananjay V. Mane

DOI:

https://doi.org/10.46243/jst.2022.v7.i03.pp97-102

Keywords:

Aceclofenac, Isocratic HPLC, Bulk drug, Pharmaceutical Ingredient.

Abstract

A simple, economical, user-friendly, precise, accurate, robust, rugged and linear analytical method developed and validated for estimation (Assay) of Aceclofenac in Active Pharmaceutical Ingredients or bulk drug form.Isocratic HPLC method developed, flow rate is 1.5 ml/min, and column used Hemochrom C18 Intsil (150mm X 4.6 mm X 5 µm), Column temperature used is 25ºC. Mobile phase used is made from 50 volume of buffer consist of 0.1 % orthophosphoric acid and 50 volumes of organic phase consist of 90% Acetonitrile. The UV detection wavelength is 275 nm. Linearity of the method was observed 50 µg/ml to 150 µg/ml. The retention time of Aceclofenac is ≈ 6.0 minute. This method was validated as per ICH guideline. (1) This method is very user-friendly and can be used for estimation of Aceclofenac in Active Pharmaceutical Ingredients and Bulk drug form because Analytical method validation of this method is successfully achieved in present study.

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Published

2022-05-22

How to Cite

S. Garad, S., & V. Mane, D. (2022). A New Simple Validated RP-HPLC-PDA Analytical Method for Estimation of Aceclofenec in Active Pharmaceutical Ingredients and Bulk Drug Form Used as Anti-Anti-Inflammatory Drug. Journal of Science & Technology (JST), 7(Special Issue 1), 97–102. https://doi.org/10.46243/jst.2022.v7.i03.pp97-102