Isolation and Characterisation of UTI Pathogens and Their Antibiotic Susceptibility

Abstract

The present study aimed to identify the causative organisms present in urine specimen and their antibiotic sensitivity profile among patients suspected with urinary tract infection (UTI). Ten mid-stream urine samples were collected from patients who suspected for UTI. Total 50 isolates were isolated by culturing on MacConkeys, Cetrimide, and Mannitol salt agar plates. Gram staining resulted that, 74% isolates were Gram negative and 34% were Gram positive. The isolates were processed further for identification, and the result reveals that, Escherichia coli aspredominant isolate which constituting 70%, followed by Enterobacter spp. (20%) and Staphylococcus aureus(10%) of total isolates. Antibiotic susceptibility and resistance result had shown that, E. coli is susceptible to Ampicillin as well as Amikacin and resistance to Ceftriaxone as well as Amoxicillin. S. aureus is susceptible Ampicillin, Amikacin as well as Ceftriaxone, and resistance toAmoxicillin. While, Enterobacter spp.showed resistance to all used antibiotics (Amikacin, Ampicillin, Ceftriaxone and Amoxicillin). Present work will be helpful for clinicians in order to improve the UTI treatment and prevent antibiotic resistance.